Techniques in Biotechnology, Molecular Biology& Biochemistry
Techniques in Biotechnology, Molecular Biology& Biochemistry, available at $44.99, has an average rating of 4.25, with 16 lectures, 1 quizzes, based on 4 reviews, and has 26 subscribers.
You will learn about Understanding Two-dimensional gel electrophoresis and its applications The Microarray; the Protein chip, how it works and what are the challenges The concept of Chromatography and the affinity Chromatography The concept of Protein staining/Protein spot detection; Coomassie Dye Staining and Silver Staining Enzyme-linked immunosorbent Assay (ELISA); its types (Direct and Indirect) and its applications The concept of Drug Designing This course is ideal for individuals who are The students/teachers of Biotechnology/Molecular Biology/Microbiology and related subjects It is particularly useful for The students/teachers of Biotechnology/Molecular Biology/Microbiology and related subjects.
Enroll now: Techniques in Biotechnology, Molecular Biology& Biochemistry
Summary
Title: Techniques in Biotechnology, Molecular Biology& Biochemistry
Price: $44.99
Average Rating: 4.25
Number of Lectures: 16
Number of Quizzes: 1
Number of Published Lectures: 16
Number of Published Quizzes: 1
Number of Curriculum Items: 17
Number of Published Curriculum Objects: 17
Original Price: $44.99
Quality Status: approved
Status: Live
What You Will Learn
- Understanding Two-dimensional gel electrophoresis and its applications
- The Microarray; the Protein chip, how it works and what are the challenges
- The concept of Chromatography and the affinity Chromatography
- The concept of Protein staining/Protein spot detection; Coomassie Dye Staining and Silver Staining
- Enzyme-linked immunosorbent Assay (ELISA); its types (Direct and Indirect) and its applications
- The concept of Drug Designing
Who Should Attend
- The students/teachers of Biotechnology/Molecular Biology/Microbiology and related subjects
Target Audiences
- The students/teachers of Biotechnology/Molecular Biology/Microbiology and related subjects
Welcome to “Techniques in Biotechnology & Molecular Biology,” a comprehensive Udemy course designed to equip you with essential skills in cutting-edge biochemical techniques. Whether you’re a student, researcher, or industry professional, this course will guide you through six key techniques crucial in the field of Biotechnology & Molecular Biology.
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Two-dimensional Gel Electrophoresis (2D-GE): Dive into the intricate world of protein separation with 2D-GE. Learn the principles, experimental setup, and data analysis techniques that make this method a powerful tool for resolving complex protein mixtures. Understand how to spot and identify proteins efficiently using this advanced electrophoretic technique.
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Affinity Chromatography: Uncover the principles of affinity chromatography and its applications in purifying biomolecules. Explore various affinity matrices, ligands, and chromatographic conditions to achieve highly specific and efficient protein purification. Master the art of designing affinity chromatography experiments tailored to your research needs.
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Microarray Technology: Harness the potential of microarray technology for high-throughput analysis of nucleic acids and proteins. Explore the design, fabrication, and applications of microarrays, enabling you to simultaneously analyze thousands of biomolecules. Gain hands-on experience in interpreting microarray data for a deeper understanding of gene expression profiling and beyond.
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Protein Staining Techniques: Delve into the world of protein staining, mastering various methods to visualize and quantify proteins. Learn popular staining techniques such as Coomassie Brilliant Blue, Silver Staining, and more. Understand the advantages and limitations of each method, enabling you to choose the most suitable approach for your specific research goals.
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Enzyme-Linked Immunosorbent Assay (ELISA): Grasp the fundamentals of ELISA, a widely used technique for detecting and quantifying specific proteins. Learn about the different ELISA formats, including direct, indirect, and sandwich assays. Acquire the skills to design and optimize ELISA experiments for precise biomarker detection and quantification.
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Drug Designing in Biochemistry: Explore the exciting realm of drug designing and its pivotal role in biochemistry. Understand the principles of rational drug design, computational methods, and structure-based drug discovery. Gain insights into target identification, lead optimization, and the overall process of developing new pharmaceutical agents.
By the end of this course, you’ll possess a diverse skill set in advanced biochemical techniques, empowering you to excel in both academic and industrial settings. Enroll now and take the next step in advancing your expertise in Biotechnology & Molecular Biology.
Course Curriculum
Chapter 1: Introduction to the Course
Lecture 1: Introduction to the Course
Chapter 2: Two-dimensional gel electrophoresis
Lecture 1: An introduction to Two Dimensional gel electrophoresis; Iso-electric focusing
Lecture 2: The second dimension of 2DE; SDS-PAGE
Chapter 3: Microarray
Lecture 1: Introduction to Microarray, the probes and its sources, and detection system
Lecture 2: Types of Protein Microarray and the challenges
Chapter 4: Protein Staining/ Protein Spot detection
Lecture 1: General Principle of Protein Staining and Coomassie Blue Staining
Lecture 2: Silver Staining
Chapter 5: Affinity Chromatography
Lecture 1: General Introduction to Chromatography and Affinity Chromatography
Lecture 2: Applications and Types of Affinity Chromatography
Chapter 6: Enzyme-linked immunosorbent assay ( ELISA)
Lecture 1: A general introduction to ELISA and the concept of Direct and Indirect ELISA
Lecture 2: Positive and negative results in ELISA
Lecture 3: Competitive ELISA; A special type of ELISA
Chapter 7: Drug Designing
Lecture 1: General Introduction to drug discovery and why we need drug discovery
Lecture 2: Steps of drug discovery and its validation
Lecture 3: How to find a suitable drug and High-throughput screening (HTS) screening
Lecture 4: Important Glossary Terms of Drug Designing
Instructors
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Dr. Ibrar Khan
Professor of Biotechnology at University of Peshawar
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- 3 stars: 0 votes
- 4 stars: 3 votes
- 5 stars: 1 votes
Frequently Asked Questions
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