The Basic Principles of Gene Cloning and DNA Analysis

The Basic Principles of Gene Cloning and DNA Analysis, available at $74.99, has an average rating of 4.2, with 43 lectures, 13 quizzes, based on 19 reviews, and has 100 subscribers.

You will learn about Gene cloning, polymerase chain reaction, and the importance of gene cloning and polymerase chain reaction. Different types of vectors used in gene cloning experiments and the respective hosts of the vectors. Extraction and purification of DNA from bacteria, animals, plants, and viruses. Different DNA manipulating enzymes and their roles. Introduction of DNA into bacterial cells. Introduction of DNA into non-bacterial cells. Obtaining a Clone of a specific Gene. This course is ideal for individuals who are This course is for biologists, biotechnologists, molecular biologists, microbiologists. etc., who have just started their education in these fields and are interested in gene cloning, recombinant DNA technology, PCR, etc. It is particularly useful for This course is for biologists, biotechnologists, molecular biologists, microbiologists. etc., who have just started their education in these fields and are interested in gene cloning, recombinant DNA technology, PCR, etc.

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Summary

Title: The Basic Principles of Gene Cloning and DNA Analysis

Price: $74.99

Average Rating: 4.2

Number of Lectures: 43

Number of Quizzes: 13

Number of Published Lectures: 43

Number of Published Quizzes: 13

Number of Curriculum Items: 58

Number of Published Curriculum Objects: 58

Number of Practice Tests: 2

Number of Published Practice Tests: 2

Original Price: $22.99

Quality Status: approved

Status: Live

What You Will Learn

Gene cloning, polymerase chain reaction, and the importance of gene cloning and polymerase chain reaction.
Different types of vectors used in gene cloning experiments and the respective hosts of the vectors.
Extraction and purification of DNA from bacteria, animals, plants, and viruses.
Different DNA manipulating enzymes and their roles.
Introduction of DNA into bacterial cells.
Introduction of DNA into non-bacterial cells.
Obtaining a Clone of a specific Gene.

Who Should Attend

This course is for biologists, biotechnologists, molecular biologists, microbiologists. etc., who have just started their education in these fields and are interested in gene cloning, recombinant DNA technology, PCR, etc.

Target Audiences

This course is for biologists, biotechnologists, molecular biologists, microbiologists. etc., who have just started their education in these fields and are interested in gene cloning, recombinant DNA technology, PCR, etc.

Gene cloning is one of the most important techniques in biotechnology and modern molecular biology. It is the same technique that was used in the Human Genome Project that successfully determined the sequences of almost all the genetic content of the chromosomes of humans. Moreover, the very first commercially available recombinant product “Insulin” was also the result of gene cloning. The technique has been used in a wide variety of studies and has great importance from the industrial point of view as well. It is, thus, important to have an idea of what gene cloning is and how it works.

This course describes the basic principles of gene cloning in a simple way and is based on the book “Gene Cloning and DNA analysis” by T.A. Brown. The course has a number of lectures on topics such as vectors used for gene cloning, purification of DNA, synthesis of recombinant DNA molecules, the introduction of DNA into living cells, selection of the transformed cells, selection of the desired recombinants, polymerase chain reaction and DNA analysis using gel electrophoresis. Each section/module has at least 1 Quiz in order to test the knowledge that you’ll gain in the respective section/module. Apart from lectures and quizzes, the course consists of a mid-term and a final term; each of which consists of a practice test and an assignment. Practice tests are of particular importance as, unlike quizzes, they are not based on the knowledge but on the application of the knowledge that you’ll gain in this course.

Course Curriculum

Chapter 1: Introduction to the Course

Lecture 1: Introduction

Chapter 2: Introduction to Gene Cloning and PCR

Lecture 1: Gene Cloning

Lecture 2: Polymerase Chain Reaction

Lecture 3: Obtaining a Pure Sample of a Gene by Cloning

Lecture 4: Obtaining a Pure Sample of a Gene by PCR

Chapter 3: Vectors for Gene Cloning

Lecture 1: Vectors for Gene Cloning: Introduction

Lecture 2: Plasmids: Basic Characteristics and Replication

Lecture 3: Plasmids: Classification and Examples

Lecture 4: Cloning Vectors Based on E. coli Plasmids

Lecture 5: Bacteriophages and Their Life Cycle

Lecture 6: Lambda Phage and M13 Phage

Lecture 7: Lambda Bacteriophage Based Vectors

Lecture 8: M13 Based Vectors

Lecture 9: Vectors for Cloning in Yeast

Chapter 4: DNA Isolation and Purification

Lecture 1: DNA Purification: Introduction

Lecture 2: Genomic DNA Purification from Bacteria

Lecture 3: Genomic DNA Preparation from Non-Bacterial Organisms

Lecture 4: Preparation of Plasmid DNA

Lecture 5: Preparation of Lambda Phage DNA

Lecture 6: Preparation of M13 Phage DNA

Chapter 5: Mid Term

Chapter 6: DNA Manipulation and DNA Manipulative Enzymes

Lecture 1: DNA Manipulation: Introduction

Lecture 2: Nucleases

Lecture 3: Restriction Endonucleases

Lecture 4: Ligases and Ligation

Lecture 5: Adding Sticky Ends to the Blunt Ends

Lecture 6: DNA Polymerases

Lecture 7: DNA Modifying Enzymes

Chapter 7: Introduction of DNA into Living Cells

Lecture 1: Introduction of Recombinant DNA Molecule into Living Cells

Lecture 2: Introduction of Plasmid-Based Vectors in Bacteria – Part I

Lecture 3: Introduction of Plasmid-Based Vectors in Bacteria – Part II

Lecture 4: Introduction of Recombinant Phage DNA into Bacteria

Lecture 5: Introduction of DNA into Non-Bacterial Cells

Chapter 8: Obtaining Clone of A specific Gene

Lecture 1: Obtaining Clone of A specific Gene: Introduction

Lecture 2: Direct Selection

Lecture 3: Construction of Libraries

Lecture 4: Methods of Clone Identification Based on DNA Probes

Lecture 5: Methods of Clone Identification Based on Translation Product

Chapter 9: DNA Analysis Using Gel Electrophoresis

Lecture 1: Gel Electrophoresis

Lecture 2: Visualizing Bands of DNA Molecules in an Agarose Gel

Chapter 10: Polymerase Chain Reaction

Lecture 1: Polymerase Chain Reaction: Introduction

Lecture 2: Primers for PCR

Lecture 3: Analyzing and Cloning PCR Products

Lecture 4: Quantitative PCR

Chapter 11: Final Term

Instructors

Hasnat Mueen
Student of Biotechnology

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4 stars: 6 votes
5 stars: 9 votes

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